Cent Eur J Public Health 2002, 10(1-2):60-65
MLEE and PFGE Characterization of Neisseria meningitidis Serogroup C and B Isolated in the Slovak Republic in 1998
- 1 National Reference Laboratory for Meningococcal Infections of the Slovak Republic, State Institute of Public Health, Bratislava, Slovak Republic
- 2 National Reference Laboratory for Meningococcal Infections of the Czech Republic, National Institute of Public Health, Prague, Czech Republic
In the Slovak Republic the incidence and mortality of invasive meningococcal disease increased after 1995 when the new meningococcal clone of Neisseria meningitidis C:2a:P1.2,P1.5, ET-1.5/37 emerged. The new clone spread between 1995 and 1998 throughout the whole country.
Morbidity of invasive meningococcal disease was 1,6/100 000 of the population and fatality reached the highest level of 23 % in the Slovak Republic in 1998. The new clone caused a new emergent epidemiological and clinical situation. The occurrence of invasive meningococcal disease caused by this clone has continually risen since 1995. In 1998 72 % of all diseases in Slovakia were caused by serogroup C. The emerging clone C:2a:Pl.2,P1.5 represented 74 % of the serogroup C isolates. Clonality and genetic diversity of 15 selected meningococcal strains causing invasive meningococcal disease was compared by multilocus enzyme electrophoresis (MLEE) and DNA macrorestriction analysis by pulsedfield gel electrophoresis (PFGE). The strains of serogroup C and B were isolated in all regions of Slovakia in 1998. The majority of isolates belong to hypervirulent clone ET-15 as determined by MLEE. By PFGE a higher degree of diversity was observed.
Klíčová slova: meningitis, Neisseria meningitidis, Slovak Republic
Zveřejněno: 1. červen 2002 Zobrazit citaci
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