Cent Eur J Public Health 2004, 12(3):119-125

Immune System Parameters in Children of Central and Eastern Europe: The Cesar Study

Timová S.1, Leonardi G.S.2, Hrubá F.3, Lochman I.4, Lochmanová A.4, Erdei E.5, Jazwiec-Kanyion B.6, Niciu E.M.7, Steerenberg P.A.8, van Loveren H.8
1 Department of Clinical Immunology, F.D.Roosevelt Hospital, Banská Bystrica, Slovak Republic
2 Environmental Epidemiology Unit, London School of Hygiene and Tropical Medicine, United Kingdom
3 Department of Informatics and Health Statistics, State Institute of Public Health, Banská Bystrica, Slovak Republic
4 Department of Immunology and Allergology, Regional Institute of Public Health, Ostrava, Czech Republic
5 Department of Biological Monitoring, J. Fodor National Public Health Center, National Institute of Environmental Health, Budapest, Hungary
6 Institute of Occupational Medicine and Environmental Health, Sosnowiec, Poland
7 Department of Environmental Medicine, Institute of Public Health, Bucharest, Romania
8 Laboratory of Pathology and Immune Biology, National Institute of Public Health and the Environment, Bilthoven, The Netherlands

Objective of this paper is to compare observed values of immune parameters obtained in the CESAR study (The Central Europe Study of Air Pollution and Respiratory Health, funded by EC PHARE program) with ranges derived from other large population-based studies.
Study design: Data were collected in healthy school children aged 9-11 years, in 6 countries: Bulgaria, the Czech Republic, Hungary, Poland, Romania and the Slovak Republic with the same standard approach in 1996. Random samples of 85 children per country, from 19 communities were selected from children having completed the health questionnaire, in total 495 children were analyzed. Lymphocyte subsets were determined by two-colour flow cytometric immunophenotyping using the lysed whole blood method (Becton-Dickinson). For determination of immunoglobulin concentration in sera nephelometric method (Behring Nephelometer system) was used.

Results: Medians, (5th-95th percentiles) of the lymphocyte subsets absolute count (x 109/l) were as follows: CD19+ B cells 0.36 (0.13-0.66), total CD3+ T cells 1.74 (0.98-2.90), CD3+CD4+ helper-inducer T cells 0.95 (0.47-1.78), CD3+CD8+ suppressor/cytotoxic T cells 0.71 (0.38-1.22), CD3-CD16+56+ NK cells 0.36 (0.14-0.78), and for CD3+CD4+/ CD3+CD8+ ratio 1.4 (0.8-2.4). Medians, (5th-95th percentiles) of percentages of lymphocyte subpopulations (%) were as follows: CD19+ B 13 (7-22), CD3+ T 70 (59-80), CD3+CD4+ T 38 (27-48), CD3+CD8+ T 28 (20-39), CD3-CD16+56+ NK cells 14 (6-27). Medians, (2.5th-97.5th percentiles) of the total immunoglobulin [g/l] were 11.7 (7.4-18.2) for IgG, 1.2 (0.5-2.5) for IgM, and 1.5 (0.5-3.4) for IgA.
Based on the aspects of the size of the CESAR immune biomarker study and on the use of the standardized protocols we recommend to use the reference ranges on lymphocyte subsets and immunoglobulin in Europe as provided by this study.

Klíčová slova: lymphocyte subsets, flow cytometry, immunoglobulin, reference intervals

Vloženo: 1. prosinec 2003; Revidováno: 5. duben 2004; Přijato: 5. duben 2004; Zveřejněno: 1. září 2004  Zobrazit citaci

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Timová S, Leonardi GS, Hrubá F, Lochman I, Lochmanová A, Erdei E, et al.. Immune System Parameters in Children of Central and Eastern Europe: The Cesar Study. Cent Eur J Public Health. 2004;12(3):119-125. PubMed PMID: 15508409.
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    Reference

    1. Bonilla FA, et al.: Normal ranges for lymphocyte subsets in children. J Pediatr 1997; 130 (3): 347-349. Přejít na PubMed...
    2. Comans-Bitter WM, de Groot R, van den Beemd R, Neijens HJ, Hop WC, Groeneveld K, et al.: Immunophenotyping of blood lymphocytes in childhood. Reference values for lymphocyte subpopulations. J Pediatr 1997; 130 (3): 388-393. Přejít k původnímu zdroji... Přejít na PubMed...
    3. Erkeller-Yuksel F, Deneys V, Yuksel B, et al.:Age-related changes in human blood lymphocyte subpopulations. J Pediatr 1992; 120:216-222. Přejít k původnímu zdroji... Přejít na PubMed...
    4. Robinson M, et al.: An analysis of the normal ranges of lymphocyte subpopulations in children aged 5-13 years. Eur J Pediatr 1996; 155 (7): 535-539. Přejít k původnímu zdroji...
    5. CESAR: Central European Study onAir Pollution and Respiratory Health. PHARE report; 1998: I. Evaluation. II. Questionnaires. III. Manuals and Protocols. IV. Study Results.
    6. Fletcher T, Brunekreef B, Fabianova E, Gurzau, ES, Houthuijs D, Lebret E, et al.: Central European Study onAir pollution and Respiratory health (CESAR): an introduction. Epidemiology 1999; 10: S29.
    7. Environmental Health Criteria 180. Principles and methods for assessing direct immune toxicity associated with exposure to chemicals. WHO, Geneva; 1996.
    8. van Loveren H, Germolec D, Lowery HS, Luster MI, Nolan C, Repetto R, et al.: Report on the Bilthoven Symposium:Advance of epidemiological studies in assessing human health aspect of immunotoxic agents in the environment and workplace. Biomarkers 1999; 4:135-157. Přejít k původnímu zdroji... Přejít na PubMed...
    9. Hannet I, Erkeller-Yuksel F, Deneys V, Lydyard P, De Bruyere M.: Lymphocyte populations as a function of age. Immunol Today 1992.
    10. Ritchie RF, Palomaki GE, Neveux LM, Navolotskaia O, Ledue TB, Craig WY.: Reference distributions for immunoglobulin A, G, and M: a practical, simple, and clinically relevant approach in a large cohort. J Clinic Lab Anal 1998; 12 (6):363-370. Přejít k původnímu zdroji...
    11. Ritchie RF, Palomaki GE, Neveux LM, Navolotskaia O.: Reference Distribution for Immunoglobulin A, G, and M: a comparison of a large cohort to the world's literature. J Clin Lab Anal 1998; 12 (6):371-377. Přejít k původnímu zdroji...
    12. McNerlan SE, Alexander HD, Rea IM.: Age-related reference intervals for lymphocyte subsets in whole blood of healthy individuals. Scand J Clin Lab Invest 1999; 59:89-92. Přejít k původnímu zdroji...
    13. The European Collaborative study.Age-related standards forTlymphocyte subsets based on uninfected children born to human immunodeficiency virus 1-infected women. Pediatr Infect Dis J 1992; 11:1018-1026. Přejít k původnímu zdroji...
    14. Heldrup J, Kalm O, Prellner K.: Blood T and B lymphocyte subpopulations in healthy infants and children. Acta Paediatr 1992; 81:125-132. Přejít k původnímu zdroji... Přejít na PubMed...
    15. Bofill M, Janossy G, Lee CA, Macdonald-Bums D, Phillips AN, Sabin C, Timms A, et al.: Laboratory control values for CD4 and CD8 T lymphocytes. Implications for HIV-1 diagnosis. Clin Exp Immunol 1992; 88:243-252. Přejít k původnímu zdroji... Přejít na PubMed...
    16. Wagner V, Wagnerová M.: Ecoimunology. Avicenum; 1988. (In Czech.)
    17. Leonardi GS, Houthuijs D, Steerenberg PA, Fletcher T, Armstrong B, Antova T.: Immune biomarkers in relation to exposure to particulate matter: a cross-sectional survey in 17 cities of Central Europe. Inhalation Toxicol 2000; 12(Supplement 4):1-14. Přejít k původnímu zdroji... Přejít na PubMed...
    18. Bain BJ.: Blood cells. A practical guide. Blackwell Science; 1995.
    19. Dallman, PR.: Blood cell normal ranges. In: Rudolph AM, editor. Pediatrics, 16th ed. New York: Appleton-Century-Crofts; 1977. p.1178.
    20. Thomas L.: Proteindiagnostik, Bestimmung von Serumproteinen, Anderung der Referenzbereiche bei Umstelung der Kalibration ab 12.9.95 CRP470. Views + Reviews, Aktuelle Labor-Information 1995; 1:1-4.
    21. Thomas L.: Referenzbereiche für Plasmaproteine. Thomas Views + Reviews, Aktuelle Labor-Information 1998; 5:1-4.
    22. Frank MM, Austen KF, Claman HN, Unanue, ER, editors.: Samter's immunology disease. 5th ed. Little Brown and Company; 1995.
    23. Bienvenu J, Whicher J,Aguzzi F.: Immunoglobulin In: Ritchie RF, Navolotskaia O. Serum proteins in clinical medicine. Scarborouhg Foundation of Blood Research 1996; 11:1-16.

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