Cent Eur J Public Health 2004, 12(3):141-150

Molecular Epidemiology of Tuberculosis in Prague and South Moravia, Czech Republic: Genetic Analysis of Mycobacterium Tuberculosis Isolates by IS6110-RFLP Fingerprinting and Spoligotyping

Kurepina N.E.1, Kreiswirth B.N.1, Shaskina E.1, Driscoll J.R.2, Polanecký V.3, Kozáková B.3, Mezenský L.4, Kubín M.3
1 Public Health Research Institute at International Center for Public Health, Newark, NJ
2 Wadsworth Center, New York State Department of Health, Albany, NY, USA
3 Institute of Hygiene of the City of Prague, Prague
4 Institute of Hygiene of the Region of South Moravia, Brno, Czech Republic

Objectives: To genetically characterize and compare Mycobacterium tuberculosis isolates among culture-confirmed TB cases in two regions in the Czech Republic in 1998.

Methods: Consecutive M. tuberculosis isolates from 111 TB patients in Prague and 120 patients in the South Moravia region were genotyped using the standardized IS6110 Southern blot hybridization method and by spoligotyping.

Results: Eighty of the Prague patients (72.1%) had isolates with unique RFLP patterns, while 31 (27.9%) had isolates which belonged to 10 clusters. Seventy-eight (64.7%) of the South Moravia strains displayed unique RFLP pattern and 42 (35.3%) were assigned into 15 clusters. The spoligotype profiles previously identified in the U.S. were found in 69 (33%) samples and newly identified Czech spoligotypes in 24 (11.4%) of the total number of examined strains.

Conclusions: The present population-based molecular epidemiological study performed in two regions of the Czech Republic in 1998 demonstrated the distribution of individual genotypes as well as clustered strains of M. tuberculosis isolated from TB patients, and confirmed the similarity between the Czech strain collection and the European Community TB Database, that includes countries with low TB rate. The sporadic import of TB cases from foreign countries and recent transmission events probably do not play significant roles in the epidemiological situation in the Czech Republic.

Received: January 5, 2004; Revised: March 16, 2004; Accepted: March 16, 2004; Published: September 1, 2004  Show citation

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Kurepina NE, Kreiswirth BN, Shaskina E, Driscoll JR, Polanecký V, Kozáková B, et al.. Molecular Epidemiology of Tuberculosis in Prague and South Moravia, Czech Republic: Genetic Analysis of Mycobacterium Tuberculosis Isolates by IS6110-RFLP Fingerprinting and Spoligotyping. Cent Eur J Public Health. 2004;12(3):141-150. PubMed PMID: 15508413.
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    References

    1. BifaniPJ,MathemaB,KurepinaNE,KreiswirthBN: Globaldissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol 2002;10:45-52. Go to original source...
    2. Dale JW, Brittain D, Cataldi AA, Cousins D, Crawford JT, Driscoll J, Heersma H, Lillebaek T, Quitugua T, Rastogi N, Skuce RA, Sola C, van Soolingen D, Vincent V: Spacer oligonucleotide typing of bacteria of the Mycobacterium tuberculosis complex: recommendations for standardised nomenclature. Int J Tuberc Lung Dis 2001;5:216-219. Go to PubMed...
    3. Driscoll JR, Bifani PJ, Mathema B, McGarry MA, Zickas GM, Kreiswirth BN, Taber HW. Spoligologos: A Bioinformatic Approach to Displaying and Analyzing Mycobacterium tuberculosis data. Emerg Infect Dis 2002;8:1306-1309. Go to original source... Go to PubMed...
    4. EuroTB (Institut de Veilee Sanitaire/kncv) and the national coordinators for tuberculosis surveillance in the WHO European Region. Surveillance of Tuberculosis in Europe. Report on tuberculosis cases notified in 1999. Saint-Maurice, France, 2002.
    5. Filliol I, Driscol J, van Sooligen D: Global distribution of Mycobacterium tuberculosis spoligotypes. Emerg Infec Dis 2002;8:1347-1349. Go to original source... Go to PubMed...
    6. Kremer K, van Sooligen D, Půtová I, Kubín M: Use of IS6110 DNA fingerprinting in tracing man-to-man transmission of Mycobacterium tuberculosis in the Czech Republic. Cent Eur J Publ Hlth 1996;4:3-6.
    7. Kubín M, Havelková M, Hynčicová I, Švecová Z, Kaustová J, Kremer K, van Soolingen D: A multidrug-resistant tuberculosis microepidemic caused by genetically closely related Mycobacterium tuberculosis strains. J Clin Microbiol 1999;37:2715-2716. Go to original source... Go to PubMed...
    8. Kubin M, Mezenský L, Polanecký V, Kremer K, van Sooligen D: Family outbreaks of tuberculosis in South Moravia low incidence area of the Czech Republic. Int J Tuberc Lung Dis 2001;5:157-159.
    9. Kubín M, Riley LW, Havelková M, Poltoratskaia N, KočováA: Molecular epidemiology of tuberculosis in Prague: analysis by restriction fragment length polymorphism. Int J Infect Dis 1998;2:155-158. Go to original source...
    10. Kubín M, Driscoll J, Polanecký V, Zdražílek J, Mezenský L: Spoligotypes of Czech populations of Mycobacterium tuberculosis. Methodology and pilot study. Epidemiol Mikrobiol Imunol 2002;51:55-59. (In Czech.)
    11. Kubín M, Fridrichovská E, Kozáková B, Zdražílek J, Kreiswirth BN: Transmission of tuberculosis from a patient to a laboratory technician confirmed by DNAfingerprinting. Epidemiol Mikrobiol Imunol 2001;50: 157-159. (In Czech.)
    12. Kubín M, Lillebaek T, Polanecký V, Kozáková B, Švecová Z, Papíková E, Vobora A. Molecular epidemiology of tuberculosis among prisoners. Epidemiol Mikrobiol Imunol 2003;52:3-8. (In Czech.)
    13. Kubín M, Polanecký V, Kozáková B, Mezenský L, Kreiswirth BN, Kurepina N, Driscoll JR: Genotype W-Beijing of Mycobacterium tuberculosis in Prague and South Moravia. Stud Pneumol Phtiseol 2003;63:115-118. (In Czech.)
    14. KurepinaNE,SreevatsanS,PlikaytisBB,BifaniPJ,ConnellND,Donnelly RJ, van Sooligen D, Musser JM, Kreiswirth BN: Characterization of the phylogenetic distribution and chromosomal insertion sites of five IS6110 elements in Mycobacterium tuberculosis: non-random integration in the dnaA-dnaN region. Tuberc Lung Dis 1998;79:31-42. Go to original source... Go to PubMed...
    15. Kurepina N et al.:The sequence analysis of the pncAgene determining the PZA-resistance in the predominant Mycobacterium tuberculosis strains isolated in the Tomsk penitentiary system, Western Siberia, Russia. Int J Tuberc Lung Dis 2001;5:S41.
    16. McNabb SJN, Braden CR, Navin TR: DNA fingerprinting of Mycobacterium tuberculosis: Lessons learned and implications for the future. Emerg Infect Dis 2002;8:1314-9. Go to original source... Go to PubMed...
    17. Mokrousov I, Narvskaya O, Otten T, Vyazovaya A, Limeschenko E, Steklova L, Vyshnevskyi B: Phylogenetic reconstruction within Mycobacterium tuberculosis Beijing genotype in northwestern Russia. Res Microbiol 2002;153:629-37. Go to original source... Go to PubMed...
    18. Narvskaya O, Otten T, Limeschenko E, Sapozhnikova N, Graschenkova O, Steklova L, Nikonova A, Filipenko ML, Mokrousov I, Vyshnevskyi B: Nosocomial outbreak of multidrug-resistant tuberculosis caused by a strain of Mycobacterium tuberculosis W-Beijing family in St. Petersburg, Russia. Eur J Clin Microbiol Infect Dis 2002;21:596-602. Go to original source... Go to PubMed...
    19. Toungoussova OS, Mariandyshev A, Bjune G, Sandven P, Caugant DA: Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates in the Archangel prison in Russia: predominance of the W-Beijing clone family. Clin Infect Dis 2003;37:665-72. Go to original source... Go to PubMed...
    20. Tuberculosis and Respiratory Diseases in 1998. Institute of Medical Information and Statistics, Ministry of Health, Prague. 1999.
    21. van Embden JD, Cave MD, Crawford JT, Dale JW, Eisenach KD, Gicquel B, Hermans P, Martin C, McAdam R, Shinnick TM, et al.: Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 1993;31:406-9. Go to original source... Go to PubMed...
    22. van Soolingen D: Molecular epidemiology of tuberculosis and other mycobacterial infections: main methodologies and achievements. J Intern Med 2001;249:1-26. Go to original source... Go to PubMed...

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