Cent Eur J Public Health 2004, 12(Supplement):S4-S7

Biochemical Effects of Low Level Exposure to Soman Vapour

Bajgar J.1, Ševelová L.1, Krejčová G.1, Fusek J.1, Vachek J.1, Kassa J.1, Herink J.2, de Jong L.P.A.2, Benschop H.2
1 Purkyně Military Medical Academy, Department of Toxicology, Hradec Králové, Czech Republic
2 TNO Prins Maurits Laboratory, Rijswijk, The Netherlands

The aim of this study was to demonstrate changes in acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities, tyrosine aminotransferase activity (TAT) and plasma corticosterone level, neuroexcitability and behavior following 24 hours and 4 weeks of soman sublethal inhalation exposure at low level. AChE activity in erythrocytes and BuChE activity in plasma was decreased (dependent on the concentration of soman) 24 h and 4 weeks after the exposure. Similar decrease in AChE activity in different brain parts was observed. One of stressogenic parameters (TAT) was changed after 24 h exposure only. 4 weeks after the exposure, these parameters (corticosterone and TAT) were in the range of normal values. Behaviour of experimental animals was changed 24 h after the exposure persisting 4 weeks after the exposure as well as neuroexcitability.

Keywords: cholinesterases, guinea pig, soman, inhalation, stressogenic markers

Published: March 1, 2004  Show citation

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Bajgar J, Ševelová L, Krejčová G, Fusek J, Vachek J, Kassa J, et al.. Biochemical Effects of Low Level Exposure to Soman Vapour. Cent Eur J Public Health. 2004;12(Supplement):S4-7. PubMed PMID: 15141961.
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    References

    1. Bajgar J: Biological monitoring of exposure to nerve agents. Brit J Ind Med 1992; 49: 648-653. Go to original source...
    2. Marrs TL: Organophosphate poisoning. Pharmacol Ther 1993; 58: 51-66. Go to original source...
    3. Brown MA, Kelley AB: Review of health consequence from high-, intermediate- and low-level exposure to organophosphorus nerve agents. J Appl Toxicol 1998; 18: 393-408. Go to original source...
    4. Kassa J, Pecka M, Tichý M, Bajgar J, Koupilová M, Herink J, Kročová Z: Toxic effect of sarin in rats at three months following single or repeated low-level inhalation exposure. Pharmacol Toxicol 2001; 88: 209-212 Go to original source... Go to PubMed...
    5. Kassa J, Koupilová M, Vachek J: The influence of low-level sarin inhalation exposure on spatial memory in rats. Pharmacol Biochem Behav 2001; 70: 175-179. Go to original source...
    6. Kassa J, Koupilová M, Herink J, Vachek J: The long term influence of low-level sarin exposure on behavioral and neurophysiological functions in rats. Acta Medica (Hradec Králové) 2001; 44: 21-27. Go to original source... Go to PubMed...
    7. Ellman GL, Courtney DK, Andres V, Featherstone RM: A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961; 7: 88-95. Go to original source... Go to PubMed...
    8. Mattingly D: A simple fluorimetric method for the estimation of free 11-hydroxycorticoids in human plasma. J Clin Pathol 1962; 15: 374-379. Go to original source...
    9. DiamondstoneTL:Assay of tyrosine transaminase activity by conversion of p-hydroxyphenylpyruvate to hydroxybenzaldehyde. Anal Biochem 1966; 16: 395-401. Go to original source...
    10. Herink J, Koupilová M, Krs O, Bajgar J: Modelling of some neuropathological states of the central nervous system by aziridine derivatives. Čs Fysiol 1992; 14 (suppl.): 7-10.
    11. Ševelová L,Vachek J, BajgarJ: Inhalation apparatus for generating sarin and soman toxic vapors. Acta Med (Hradec Králové), 2003, in press. Go to original source...
    12. Bajgar J, Fusek J, Kassa J., Krejčová G, Ševelová L: Biochemical effects of low level exposure to soman vapour. NBC 2003. Nuclear, Biological and Chemical Threats - a Crisis Management Challenge. 15-18 June 2003, Jyvaskyla, Finland. Symposium Proceedings, pp. 167-168.
    13. Bajgar J, Fusek J, Herink J: Toxicity assessment of highly toxic organophosphates in humans extrapolated from experimental data in vitro and in vivo. Proceedings of the CB Medical Treatment Symposium: An Extrapolation of Present Capabilities and Future Requirements for CBMT. 7-12 July 1996, Spiez, Switzerland, pp. 205-209.
    14. Benschop H, De Jong LPA: Toxicokinetics of nerve agents. In: Chemical Warfare Agents: Toxicity at Low Levels (Somani SM and Romano JA, Eds), Chapter 2, CRC Presss, Boca Raton, Florida, USA, 2001. Go to original source...
    15. Kassa J, Bajgar J: In vitro inhibition of rabbit and guinea pig erythrocyte acetylcholinesterase by soman. Toxicol In Vitro 1999; 13: 403-408. Go to original source...
    16. Scremin OU, Shih T-M, Huynh L, Roch M, Booth R, Jenden DJ: Delayed neurologic and behavioural effects of subtoxic doses of cholinesterase inhibitors. J Pharmacol Exp Therap 2003; 304: 1111-1119. Go to original source...
    17. Morita H, Yanagisawa T, Nakajima M, Shimizu M, Hirabayashi H, Okudera H, Nohara M, Midorikawa Y, Mimura S: Sarin poisoning in Matsumoto, Japan Lancet 1995; 346: 290-293. Go to original source...
    18. Kassa J: Non-specific affetcs of organophosphorus inhibitors of cholinesterases. Voj Zdrav Listy Suppl. 2 1998; 67: 15-19.
    19. Kassa J, Franková K, Hoder P, Patočka J:Acomparison of the efficacy of cholinolytics atropine and biperiden (Akineton) in combination with HI-6 on cholinergic and stressogenic effects of soman in rats. Homeostasis 1996; 37: 135-136.

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