Cent Eur J Public Health 2004, 12(Supplement):S11-S13
Some Lung Cellular Parametres Reflecting Inflammation after Combined Inhalation of Amosite Dust with Cigarette Smoke by Rats
- 1 Slovak Medical University, Institute of Preventive and Clinical Medicine Bratislava, Slovak Republic
- 2 Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece
Cellular changes were followed in lung cell suspensions after 175 day inhalation by rats of concentrations 30 mg/m3 or 60 mg/m3 of amosite asbestos every second day combined with daily exposure to cigarette smoke at 30 mg of total particulate matter (TPM)/m3 air. Concomitantly, lung inflammation was assessed by changes in the bronchoalveolar lavage fluid (BALF). A dose-dependent rise in the BALF inflammatory parameters was found. The rise of the proportion of binucleate (BNC) and multinucleate cells (MNC) in lung cell suspensions was also dose-dependent. It is concluded that, in the experimental assessment of effects of fibrogenic dusts, the number of BNC and of MNC in lung cell suspensions may serve as a useful semiquantitative biomarker of the inflammation.
Klíčová slova: amosite, asbestos, inhalation, rats, multinucleate cells
Zveřejněno: 1. březen 2004 Zobrazit citaci
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