Cent Eur J Public Health 2004, 12(Supplement):S62-S64

Inhibitory Effects of Cadmium and Tabacco Alkaloids on Expansion of Porcine Oocytecumulus Complexes

Mlynarčíková A.1, Scsuková S.1, Vršanská S.1, Nagyová E.2, Ficková M.1, Kolena J.1
1 Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic
2 Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic

Studies aimed at the influence of smoking on reproductive functions have found out fertility disorders in smokers occurring at any stage of reproductive processes. In our experiments the role of cadmium, nicotine and anabasine was investigated in the expansion of oocyte-cumulus complexes (OCC) isolated from large antral porcine follicles. Suppression of FSH-induced cumulus expansion and significant inhibition of synthesis and accumulation of hyaluronic acid in the cell/matrix compartment of the OCC was observed in the presence of different concentrations of tested compounds. The suppressive effect of cadmium and tobacco alkaloids on the cumulus expansion was accompanied by decreased progesterone production by cumulus cells during 42 h incubation of the OCC with FSH.

Klíčová slova: porcine follicles, cumulus expansion, hyaluronic acid, progesterone, tobacco alkaloids

Zveřejněno: 1. březen 2004  Zobrazit citaci

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Mlynarčíková A, Scsuková S, Vršanská S, Nagyová E, Ficková M, Kolena J. Inhibitory Effects of Cadmium and Tabacco Alkaloids on Expansion of Porcine Oocytecumulus Complexes. Cent Eur J Public Health. 2004;12(Supplement):S62-64. PubMed PMID: 15141982.
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    Reference

    1. Zenzes MT: Smoking and reproduction: gene damage to human gametes and embryos. Hum Reprod Update 2000; 6(2): 122-131. Přejít k původnímu zdroji...
    2. De Felici M, SalustriA, Siracusa G: Spontaneous hardening of the zona pellucida of mouse oocytes duringin vitro culture. II.The effect of follicular fluid and glycosaminoglycans. Gamete Res 1985; 12: 227-235. Přejít k původnímu zdroji...
    3. Ježová M, Scsuková S, Nagyová E, Vranová J, Procházka R, Kolena J: Effect of intraovarian factors on porcine follicular cells: cumulus expansion, granulosa and cumulus cell progesterone production. Anim Reprod Sci 2001; 65: 115-126. Přejít k původnímu zdroji...
    4. Nagyová E, Procházka R, Vanderhyden BC: Oocytectomy does not influence synthesis of hyaluronic acid by pig cumulus cells: retention of hyaluronic acid after insulin-like growth factor I treatment in serum-free medium. Biol Reprod 1999; 61: 569-574. Přejít k původnímu zdroji... Přejít na PubMed...
    5. Paksy K, Rajczy K, Forgacs Z, Lazar P, BernardA, Gati I, Kaali GS: Effect of cadmium on morphology and steroidogenesis of cultured human ovarian granulosa cells. J Appl Toxicol 1997; 18 (5): 321-327. Přejít k původnímu zdroji...
    6. Blackburn CW, Peterson CA, Hales HA, Carrell DT, Jones KP, Urry RL, Peterson CM: Nicotine, but not cotinine, has a direct toxic effect on ovarian function in the immature gonadotropin-stimulated rat. Reprod Toxicol 1994; 8: 325-331. Přejít k původnímu zdroji... Přejít na PubMed...
    7. Gocze PM, Szabo I, Freeman DA: Influence of nicotine, cotinine, anabasine and cigarette smoke extract on human granulosa cell progesterone and estradiol synthesis. Gynecol Endocrinol 1999; 13: 266-272. Přejít k původnímu zdroji...
    8. Barbieri RL, McShane PM, Kenneth JR: Constituents of cigarette smoke inhibit human granulosa cell aromatase. Fertil Steril 1986; 46: 232-236. Přejít k původnímu zdroji...

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