Cent Eur J Public Health 2004, 12(Supplement):S62-S64

Inhibitory Effects of Cadmium and Tabacco Alkaloids on Expansion of Porcine Oocytecumulus Complexes

Mlynarčíková A.1, Scsuková S.1, Vršanská S.1, Nagyová E.2, Ficková M.1, Kolena J.1
1 Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic
2 Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Liběchov, Czech Republic

Studies aimed at the influence of smoking on reproductive functions have found out fertility disorders in smokers occurring at any stage of reproductive processes. In our experiments the role of cadmium, nicotine and anabasine was investigated in the expansion of oocyte-cumulus complexes (OCC) isolated from large antral porcine follicles. Suppression of FSH-induced cumulus expansion and significant inhibition of synthesis and accumulation of hyaluronic acid in the cell/matrix compartment of the OCC was observed in the presence of different concentrations of tested compounds. The suppressive effect of cadmium and tobacco alkaloids on the cumulus expansion was accompanied by decreased progesterone production by cumulus cells during 42 h incubation of the OCC with FSH.

Keywords: porcine follicles, cumulus expansion, hyaluronic acid, progesterone, tobacco alkaloids

Published: March 1, 2004  Show citation

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Mlynarčíková A, Scsuková S, Vršanská S, Nagyová E, Ficková M, Kolena J. Inhibitory Effects of Cadmium and Tabacco Alkaloids on Expansion of Porcine Oocytecumulus Complexes. Cent Eur J Public Health. 2004;12(Supplement):S62-64. PubMed PMID: 15141982.
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