Cent Eur J Public Health 2006, 14(4):168-174 | DOI: 10.21101/cejph.a3389

Molecular Epidemiology of Tuberculosis in the Czech Republic, 2004: Analysis of M. tuberculosis Complex Isolates Originating from the City of Prague, South Moravia and the Moravian-Silesian Region

Wolfgang M. Prodinger1, Vladimír Polanecký2, Bohumila Kozáková3, Maria Müllerová4, Lev Mezenský5, Jarmila Kaustová6, Carolin Lechleitner1, Milan Kubín2
1 Dept. of Hygiene, Microbiology and Social Medicine, Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria
2 Institute of Hygiene of the City of Prague, Czech Republic
3 Metropolitan Institute of Public Health, Prague, Czech Republic
4 KlinLab s. r. o. Prague, Czech Republic
5 Regional Institute of Public Health, Brno, Czech Republic
6 Regional Institute of Public Health, Ostrava, Czech Republic

Objectives: To compare M. tuberculosis complex genotypes from representative regions of the Czech Republic in order to estimate changes in strain prevalence and in the extent of imported drug-resistant tuberculosis.

Methods: Primary M. tuberculosis complex isolates (n = 155) and follow-up isolates (n = 15) from 155 patients from the first half of 2004 (98 from Prague, 37 from South Moravia and 35 from the Moravian-Silesian region) were genotyped by IS6110-RFLP, spoligotyping, and partly by VNTR-genotyping.

Results: Based on IS6110-RFLP, 110 of 155 (71%) primary isolates were unique. Forty-five isolates (29 %) were found in 15 clusters comprising two to six patients and all but one cluster were also discriminated by MIRU-VNTR-genotyping. Four clusters comprised patients from different regions, and six were ongoing for several years. An indication of MDR-strain transmission was found in one instance. All four Beijing-type isolates with any resistance were associated with immigration from Eastern Europe.

Conclusions: The molecular epidemiological data of this period-prevalence, population based study and its comparison to earlier investigations point to a low extent of clustering between M. tuberculosis complex isolates in representative regions of the Czech Republic. Few clusters extending geographically and/or over several years were identified, providing a means for an in-depth analysis of risk factors of transmission. Beijing genotype isolates were shown to increase in prevalence to reach 6.5%. Drug resistant isolates of this genotype were associated with immigration of from Eastern Europe, although direct transmission of a resistant isolate was probable only in one of eleven cases.

Keywords: tuberculosis, fingerprinting, epidemiology, Beijing-genotype

Received: March 16, 2006; Revised: June 30, 2006; Accepted: June 30, 2006; Published: December 1, 2006  Show citation

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Prodinger WM, Polanecký V, Kozáková B, Müllerová M, Mezenský L, Kaustová J, et al.. Molecular Epidemiology of Tuberculosis in the Czech Republic, 2004: Analysis of M. tuberculosis Complex Isolates Originating from the City of Prague, South Moravia and the Moravian-Silesian Region. Cent Eur J Public Health. 2006;14(4):168-174. doi: 10.21101/cejph.a3389. PubMed PMID: 17243495.
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