Cent Eur J Public Health 2007, 15(1):38-43 | DOI: 10.21101/cejph.a3401

First Confirmation of Bordetella pertussis Occurence in Slovakia by Using Real-time PCR

Lucia Maďarová1, Cyril Klement1, Desana Kohútová1, Katarína Tináková2, Ľudmila Krajčíková3, Margita Obernauerová4
1 Regional Authority of Public Health Banská Bystrica, Division of Medical Microbiology, Banská Bystrica, Slovak Republic
2 Regional Authority of Public Health Nitra, Division of Epidemiology, Nitra, Slovak Republic
3 Regional Authority of Public Health Topoľčany, Division of Epidemiology, Topoľčany, Slovak Republic
4 Faculty of Natural Sciences, Comenius University, Department of Microbiology and Virology, Bratislava, Slovak Republic

By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis - pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections.

Keywords: Bordetella pertussis, whooping cough, real-time PCR, LightCycler

Received: June 8, 2006; Revised: October 17, 2006; Accepted: October 17, 2006; Published: March 1, 2007  Show citation

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Maďarová L, Klement C, Kohútová D, Tináková K, Krajčíková Ľ, Obernauerová M. First Confirmation of Bordetella pertussis Occurence in Slovakia by Using Real-time PCR. Cent Eur J Public Health. 2007;15(1):38-43. doi: 10.21101/cejph.a3401. PubMed PMID: 17491558.
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    References

    1. Onorato IM, Wassilak SG. Laboratory diagnosis of pertussis: the state of the art. Pediatr Infect Dis J. 1987 Feb;6(2):145-51. Go to original source... Go to PubMed...
    2. Kerr JR, Matthews RC. Bordetella pertussis infection: pathogenesis, diagnosis, management, and the role of protective immunity. Eur J Clin Microbiol Infect Dis. 2000 Feb;19(2):77-88. Go to original source...
    3. Hourd S, Hackelc C, Herzog A, Bollen A. Specific identification of Bordetella pertussis by the polymerase chain reaction. Res Microbiol. 1989 Sep;140(7):477-87. Go to original source... Go to PubMed...
    4. Mastrantonio P, Stefanelli P, Giuliano M. Polymerase chain reaction for the detection of Bordetella pertussis in clinical nasopharyngeal aspirates. J Med Microbiol. 1996 Apr;44(4):261-6. Go to original source... Go to PubMed...
    5. Glare EM, Paton JC, Premier RR, Lawrence AJ, Nisbet IT. Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction. J Clin Microbiol. 1990 Sep;28(9):1982-7. Go to original source... Go to PubMed...
    6. Douglas E, Coote JG, Parton R, McPheat W. Identification of Bordetella pertussis in nasopharyngeal swabs by PCR amplification of a region of the adenylate cyclase gene. J Med Microbiol. 1993 Feb;38(2):140-4. Go to original source... Go to PubMed...
    7. Li Z, Jansen DL, Finn TM, Halperin SA, Kasina A, O'Connor SP, et al. Identification of Bordetella pertussis infection by shared-primer PCR. J Clin Microbiol. 1994 Mar;32(3):783-9. Go to original source... Go to PubMed...
    8. Olcen P, Backman A, Johansson B, Esbjorner E, Torquist E, Bygraves, J., et al. Amplification of DNA by the polymerase chain reaction for the efficient diagnosis of pertussis. Scand J Infect Dis. 1992;24(3):339-45. Go to original source...
    9. Kosters K, Riffelmann M, Wirsing von Konig CH. Evaluation of a realtime PCR assay for detection of Bordetella pertussis and B. parapertussis in clinical samples. J Med Microbiol. 2001 May;50(5):436-40. Go to original source... Go to PubMed...
    10. Reischel U, Kosters K, Leppmeier B, Linde HJ, Lehn N. Rapid Detection and Simultaneous Differentiation of Bordetella pertussis and Bordetella parapertussis in Clinical Specimans by LightCycler PCR. In: Reischl U, Wittwer C, Cockerill F, editors. Rapid Cycle Rel-Time PCR-Methods and Applications. Berlin: Springer Verlag; 2002. p. 31-43. Go to original source...
    11. Wadowski RM, Laus S, Libert T, States SJ, Ehrlich GD. Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab. J Clin Microbiol. 1994 Apr;32(4):1054-7. Go to original source... Go to PubMed...
    12. Roche Diagnostics GmbH. High Pure PCR Template Preparation Kit. Instruction Manual. March 2002.
    13. Roche Diagnostics GmbH. LightCycler Fast Start DNA Master Hybridization Probes. Instruction Manual. November 2001.
    14. Roche Diagnostics GmbH. PCR Applications Manual. 2nd ed. Mannheim: 1999.
    15. Birkebaek NH, Heron I, Skjodt K. Bordetella pertussis diagnosed by polymerase chain reaction. Bordetella pertussis diagnosed by polymerase chain reaction. APMIS. 1994 Apr;102(4):291-4. Go to original source... Go to PubMed...
    16. Fry NK, Tzivra O, LiYT, McNiffA, Doshi N, Maple PA, et al. Laboratory diagnosis of pertussis infections: the role of PCR and serology. J Med Microbiol. 2004 Jun;53:519-25. Go to original source... Go to PubMed...
    17. Storsaeter J. Studies of the protective efficacy of two acellular pertussis vaccines. Stockholm: Thesis, 1991.
    18. Žúrek D. Usefulness of real-time PCR in laboratory diagnosis. Laboratórna diagnostika. 2003;8(1):50-1. (In Czech.)
    19. He Q, Mertsola J, Soini H, Skurnik M, Ruuskanen O, Viljanen MK. Comparison of polymerase chain reaction with culture and enzyme immunoassay for diagnosis of pertussis. J Clin Microbiol. 1993 Mar;31(3):6425. Go to original source...
    20. Lind-Brandberg L, Welinder-Olsson C, Lagergard T, Taranger J, Trollfors B, Zackrisson G. Evaluation of PCR for diagnosis of Bordetella pertussis and Bordetella parapertussis infections. J Clin Microbiol. 1998 Mar;36(3):679-83. Go to original source... Go to PubMed...
    21. Yih WK, Silva EA, Ida J, Harrington N, Lett SM, George H. Bordetella holmesii-like organisms isolated from Massachusetts patients with pertussis-like symptoms. Emerg Infect Dis. 1999 May-Jun;5(3):441-3. Go to original source... Go to PubMed...
    22. Loeffelholz MJ, Thompson CJ, Long KS, Gilchrist MJ. Detection of Bordetella holmesii using Bordetella pertussis IS481 PCR assay. J Clin Microbiol. 2000 Jan;38(1):467. Go to original source... Go to PubMed...
    23. Reischl U, Lehn N, Sanden GN, Loeffelholz MJ. Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii. J Clin Microbiol. 2001 May;39(5):1963-6. Go to original source... Go to PubMed...
    24. Mazengia E, Silva EA, Peppe JA, Timperi R, George H. Recovery of Bordetella holmesii from patients with pertussis-like symptoms: use of pulsed-field gel electrophoresis to characterize circulating strains. J Clin Microbiol. 2000 Jun;38(6):2330-3. Go to original source... Go to PubMed...
    25. Dókušová L, Sirági P, Klement C, Schréter I, Kristian P, Jarčuška P., et al. Confirmation of anthrax occurrence using real-time PCR. Biologia, Bratislava. 2004;59(6):797-801.

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