Cent Eur J Public Health 2008, 16(Supplement):S52
HPV-16 DNA Viral Load Is a Strong Marker to Predict the Development of Cervical Cancer in Korean Women
- 1 Division of AIDS, Center for Immunology and Pathology, National Institute of Health, Seoul, Korea
- 2 Department of Obstetrics and Gynecology, College of Medicine, Hanyang University, Seoul, Korea
Background: In human papillomavirus (HPV)-associated carcinogenesis, HPV-16 DNA viral loads may play an important role in the progression of cervical cancer.
Objectives: This study investigated HPV-16 DNA viral load as well as epidemiological factors which could be a surrogate marker for developing cervical intraepithelial neoplasia (CIN) and cervical cancer in Korean women.
Materials and Methods: Sixty-five HPV-infected women from Hanyang University were enrolled in this study. There were 21 cases with abnormal cytology (no CIN), 5 cases with low-grade CIN (CIN1), 3 cases with high-grade CIN (CIN2/3), and 36 cases with cervical cancer. HPV-16 DNA viral load was measured with commercial HPV-16 real-time PCR kit (Primer Design Ltd).
Results: HPV-16 DNA viral load (Log10 HPV-16 DNA copies/µg DNA) significantly increased with the grade of cervical lesions as follows: 5.6 in no CIN, 6.1 in CIN1, 6.6 in CIN2/3, and 7.1 in cancer. HPV-16 DNA viral load increased with the grade of cervical cancer (6.3 in grade 1, 7.5 in grade 2, and 8.5 in grade 3). Although the HPV-16 DNA viral load did not show the significant differences for different grades of CIN, it showed the strong relationship with cervical cancer (p < 0.001). Integrated HPV-16 DNA viral load showed the significant differences between cervical cancer and no CIN (p = 0.03). The cervical disease progression increased with age (p = 0.05) but no significant association was observed with any other risk factors such as pregnancy, age of the first sexual intercourse, and number of lifetime sexual partners.
Conclusions: Our data show that HPV-16 DNA viral load might be a useful predictor for the development of CIN and cervical cancer in Korean women.
Keywords: HPV-16 DNA, HPV-16 real-time PCR, cervical cancer, cervical intraepithelial neoplasia
Published: April 1, 2008 Show citation
