Cent Eur J Public Health 2008, 16(Supplement):S59-S60

Activity and Distribution of Cells Infiltrating TC-1 Tumours after Chemotherapy and Subsequent IL-12 Immunotherapy

Marie Indrová¹, Elzbieta Pajtasz-Piasecka², Joanna Rossowska², Jana Bieblová¹, Milan Reiniš¹

¹ Institute of Molecular Genetics AS CR, v.v.i., Prague 4, Czech Republic

² Institute of Immunology and Experimental Therapy, Polish Acad. Sci., Wroclaw, Poland

Methods: Moderately immunogenic HPV-16-associated TC-1 tumour mimicking human HPV-16-associated neoplasms was used to examine the effect of local interleukin-12 gene therapy with a genetically modified tumour cell vaccine for the treatment of minimal residual tumour disease, obtained after cytoreductive chemotherapy (CMRTD) with ifosfamide derivative CBM-4A, on the distribution and activity of tumour-infiltrating cells.

Results: After chemotherapy, histological and immunohistological examinations showed a decrease or disappearance of CD4+ and CD8+ T cells as well as macrophages. The administration of the vaccine led to the abundance of macrophages and renewal of CD8+ and CD4+ cells in the tumour nodules. The FACS analysis of tumour-infiltrating cells showed a significant increase in CD11c+ cells after chemotherapy and subsequent immunotherapy. Moreover, CD45+ tumour-infiltrating cells isolated from the treated animals exhibited, after short-term in vitro precultivation, renewed cytotoxic and proliferation potential.

Conclusion: These findings contribute to the relevance of the treatment of CMRTD with genetically modified cellular vaccines.

Zveřejněno: 1. duben 2008  Zobrazit citaci