Cent Eur J Public Health 2009, 17(4):179-182 | DOI: 10.21101/cejph.b0015

Direct Detection of Borrelia burgdorferi Spirochetes in Patients with Early Disseminated Lyme Borreliosis

Katarína Schwarzová1, Zina Košťanová2, Katarína Holečková3, Eva Špitalská1, Vojtech Boldiš1
1 Institute of Virology, Slovak Academy of Sciences, Bratislava
2 Regional Office of Public Health, Žiar nad Hronom
3 Slovak Health University, Bratislava, Slovak Republic

The detection of spirochetes in 15 patients with clinically documented early disseminated LB has been analysed when using cultivation method of the plasma or the cerebrospinal fluid, electron microscopy, commercial Western blot and detecting the DNA of the pathogen in vitro cultures by PCR-RFLP. Spirochetes were isolated in eight blood and one cerebrospinal fluid culture samples. In seven cases (47%), previous serodiagnostic laboratory tests were negative. Borrelial DNA was detected by PCR in 67% patients (9 blood samples and 1 CSF sample). Using MseI restriction fragments of PCR products of the amplified rrf-rrl region, we identified Borrelia garinii (80%), one B. afzelii isolate and one B. burgdorferi s.s.

Keywords: Borrelia burgdorferi, direct detection, cultivation, electron microscopy, polymerase chain reaction (PCR), Restriction fragment length polymorphism (RFLP) analysis

Received: January 5, 2009; Revised: April 27, 2009; Accepted: April 27, 2009; Published: December 1, 2009  Show citation

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Schwarzová K, Košťanová Z, Holečková K, Špitalská E, Boldiš V. Direct Detection of Borrelia burgdorferi Spirochetes in Patients with Early Disseminated Lyme Borreliosis. Cent Eur J Public Health. 2009;17(4):179-182. doi: 10.21101/cejph.b0015. PubMed PMID: 20377044.
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