Cent Eur J Public Health 2013, 21(3):165-170 | DOI: 10.21101/cejph.a3881

Effect of TiO2 Nanofibres on Selected Bronchoalveolar Parameters in Acute and Subacute Phase - Experimental Study

Marta Hurbánková1, Silvia Černá1, Zuzana Kováčiková1, Soňa Wimmerová1, Dominika Hrašková1, Jana Marcišiaková2, Štefánia Moricová1
1 Slovak Medical University, Faculty of Public Health, Laboratory of Respiratory Toxicology, Bratislava, Slovakia
2 Medical-Preventive Limited Liability Company, Hnusta, Slovakia

Titanium dioxide nanofibres (TiO2) were intratracheally instilled in dose of 4 mg/0.2 mL saline solution per animal (Wistar rats). After 48 hours and 14 days the animals were exsanguinated (under i.p. thiopental narcosis), bronchoalveolar lavage (BAL) was perfomed and cells from BAL fluid were isolated. Following inflammatory, cytotoxic and oxidative stress BAL parameters were examined: differential cell count (% of alveolar macrophages (AM), polymorphonuclears and lymphocytes); the viability and phagocytic activity of AM; the proportion of immature cells; the proportion of multinucleated cells; count of AM/mL lavage; count of BAL cells/ mL lavage; the level of ascorbic acid and activity of superoxide dismutase, both in tissue homogenate and in bronchoalveolar lavage fluid. The majority of examined BAL parameters in the acute and subacute phase in our study suggest serious inflammatory and cytotoxic processes in lung after exposure to TiO2.

Klíčová slova: TiO2 nanofibres, inflammatory and cytotoxic parameters, bronchoalveolar lavage, intratracheal instillation

Vloženo: 19. listopad 2012; Revidováno: 19. červen 2013; Přijato: 19. červen 2013; Zveřejněno: 1. září 2013  Zobrazit citaci

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Hurbánková M, Černá S, Kováčiková Z, Wimmerová S, Hrašková D, Marcišiaková J, Moricová Š. Effect of TiO2 Nanofibres on Selected Bronchoalveolar Parameters in Acute and Subacute Phase - Experimental Study. Cent Eur J Public Health. 2013;21(3):165-170. doi: 10.21101/cejph.a3881. PubMed PMID: 24344544.
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    Reference

    1. Allhoff F, Lin P, Moore D. What is nanotechnology and why does it matter?: from science to ethics. Chichester: Wiley-Blackwell; 2010. Přejít k původnímu zdroji...
    2. Donaldson K, Borm P, editors. Particle toxicology. Boca Raton: CRC Press/Taylor & Francis Group; 2007.
    3. Yu Ming-Ho. Environmental toxicology: biological and health effects of pollutants. 2nd ed. Boca Raton: CRC Press; 2005.
    4. NelA, Xia T, Mädler L, Li N. Toxic potential of materials at the nanolevel. Science. 2006 Feb 3;311(5761):622-7. Přejít k původnímu zdroji... Přejít na PubMed...
    5. Oberdörster G, Maynard A, Donaldson K, Castranova V, Fitzpatrick J, Ausman K, et al.; ILSI Research Foundation/Risk Science Institute Nanomaterial Toxicity Screening Working Group. Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Part Fibre Toxicol. 2005 Oct 6;2:8. Přejít k původnímu zdroji... Přejít na PubMed...
    6. Borm PJA, Robbins D, Haubold S, Kuhlbusch T, Fissan H, Donaldson K, et al. The potential risks of nanomaterials: a review carried out for ECETOC. Part Fibre Toxicol. 2006 Aug 14;3:11. Přejít k původnímu zdroji... Přejít na PubMed...
    7. Warheit DB, Brock WJ, Lee KP, Webb TR, Reed KL. Comparative pulmonary toxicity inhalation and instillation studies with different TiO2 particle formulations: impact of surface treatments on particle toxicity. Toxicol Sci. 2005 Dec;88(2):514-24. Přejít k původnímu zdroji... Přejít na PubMed...
    8. Warheit DB, Webb TR, Reed KL, Frerichs S, Sayes CM. Pulmonary toxicity study in rats with three forms of ultrafine-TiO2 particles: dif ferential responses related to surface properties. Toxicology. 2007 Jan 25;230(1):90-104. Přejít k původnímu zdroji... Přejít na PubMed...
    9. Baan R, Straif K, GrosseY, Secretan B, El Ghissassi F, Cogliano V; WHO InternationalAgency for Research on Cancer MonographWorking Group. Carcinogenicity of carbon black, titanium dioxide, and talc. Lancet Oncol. 2006 Apr;7(4):295-6. Erratum in: Lancet Oncol. 2006 May;7(5):365. Přejít k původnímu zdroji...
    10. IARC Working Group on the Evaluation of Carcinogenic Risks to Humans. Carbon black, titanium dioxide, and talc. IARC Monogr Eval Carcinog Risks Hum. 2010;93:1-413.
    11. Hurbánková M, Kaiglová A. Compared effects of asbestos and wollastonite fibrous dusts on various biological parameters measured in bronchoalveolar lavage fluid. J Trace and Microprobe Techniques. 1999;17(2):233-43.
    12. Beno M, Hurbankova M, Dusinska M, Cerna S, Volkovova K, Staruchova M, et al. Multinucleate cells (MNC) as sensitive semiquantitative biomarkers of the toxic effect after experimental fibrous dust and cigarette smoke inhalation by rats. Exp Toxicol Pathol. 2005 Aug;57(1):77-87. Přejít k původnímu zdroji... Přejít na PubMed...
    13. Trouiller B, Reliene R, WestbrookA, Solaimani P, Schiestl RH. Titanium dioxide nanoparticles induce DNAdamage and genetic instability in vivo in mice. Cancer Res. 2009 Nov 15;69(22):8784-9. Přejít k původnímu zdroji... Přejít na PubMed...
    14. BenckoV,Vostal J.Air pollution by solid particles and public health: when can we conclude on causality. Cent Eur J Public Health. 1999;7(2)64-6.
    15. Murashov V, Howard J. Essential features for proactive risk management. Nat Nanotechnol. 2009 Aug;4(8):467-70. Přejít k původnímu zdroji... Přejít na PubMed...
    16. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health. Occupational exposure to titanium dioxide. Current intelligence bulletin 63. Cincinatti: Department of Health and Human Services; 2011.
    17. Dziedzic D, Wheeler CS, Gross KB. Bronchoalveolar lavage: detecting markers of lung injury. In: Corn M, editor. Handbook of hazardous materials. New York: Academic Press; 1993. p. 99 - 111. Přejít k původnímu zdroji...
    18. Gardner DE, editor. Toxicology of the lung. Boca Raton: CRC/Taylor & Francis; 2006.
    19. Hurbánková M, Kaiglová A, Buchancová J. Cytokines - the important biomarkers of lung injury after exposure to industrial fibrous dusts. Acta Medica Martiniana. 2001;1(1):19-24.
    20. Tarkowski M, Górski P. Macrophage activity in asbestos related diseases. Pol J Occup Med Environ Health. 1991;4(2):115-25. Přejít na PubMed...
    21. Hurbánková M, Tátrai E, Černá S, Six E, Kováčiková Z, Kyrtopoulos S. Inflammatory and cytotoxic effects as well as histological findings of selected industrial fibrous dusts in Fischer rats after intratracheal instillation. Biologia. 2004;59(6):769-78.

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