Cent Eur J Public Health 2016, 24(4):333-336

Should Enteroviruses Be Monitored in Natural Recreational Waters?

Soňa Šarmírová1, Viera Nagyová2, Darina Štípalová1, Iveta Drastichová2, Danka Šimonyiová3, Zuzana Sirotná3, Renáta Kissová4, Katarína Pastuchová5, Jana Tirpáková6, Daniel Kuba6, Cyril Klement4,7, Shubhada Bopegamage1
1 Enterovirus Laboratory, Faculty of Medicine, Slovak Medical University, Bratislava, Slovak Republic
2 Department of Biology, Public Health Authority of the Slovak Republic, Bratislava, Slovak Republic
3 Department of Medical Microbiology, Public Health Authority of the Slovak Republic, Bratislava, Slovak Republic
4 Department of Medical Microbiology, Regional Authority of Public Health, Banská Bystrica, Slovak Republic
5 National Reference Centre for Poliomyelitis, Public Health Authority of the Slovak Republic, Bratislava, Slovak Republic
6 National Transplant Organization, Bratislava, Slovak Republic
7 Faculty of Public Health, Slovak Medical University, Bratislava, Slovak Republic

Enteroviruses (EVs) infections occur worldwide. Although, infections by these viruses are often asymptomatic and go unnoticed, they can be shed in stool for several weeks. The EVs are associated with sporadic outbreaks and a wide range of clinical symptoms, occasionally accompanied with fatal consequences. Presently in the Slovak Republic (SR) recreational waters are tested only for bacterial indicators. Our aim was to monitor EVs in recreational waters. Water samples were collected during the years 2012-2014 from different recreational natural lakes in Central and West regions of SR. The samples were concentrated by centrifugation using the two-phase separation method recommended by the World Health Organization (WHO) used for EVs surveillance in the treated sewage waste water. Each of the two phases collected from the samples was analysed by polymerase chain reaction for detection of EVs and primary sequencing was done. Our study demonstrated presence of EVs in three localities consecutively for three years, indicating a probability of constant local source of faecal contamination. This is the first monitoring report on the occurrence of EVs in the natural recreational waters in SR.

Keywords: enteroviruses, natural recreational waters, monitoring

Received: April 1, 2015; Revised: May 27, 2016; Accepted: May 27, 2016; Published: December 1, 2016  Show citation

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Šarmírová S, Nagyová V, Štípalová D, Drastichová I, Šimonyiová D, Sirotná Z, et al.. Should Enteroviruses Be Monitored in Natural Recreational Waters? Cent Eur J Public Health. 2016;24(4):333-336. PubMed PMID: 28095292.
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