Cent Eur J Public Health 2017, 25(2):163-167

Quinolone-resistant Escherichia coli in Poultry Farming

Kristýna Hricová1, Magdaléna Röderová1, Vendula Pudová1, Vojtěch Hanulík1, Dana Halová3, Pavla Julínková3, Monika Dolejská3, Ivo Papoušek3, Jan Bardoň1,2
1 Department of Microbiology, Faculty of Medicine and Dentistry, Palacký University Olomouc, Olomouc, Czech Republic
2 State Veterinary Institute, Olomouc, Czech Republic
3 Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic

Increasing bacterial resistance to quinolone antibiotics is apparent in both humans and animals. For humans, a potential source of resistant bacteria may be animals or their products entering the human food chain, for example poultry. Between July 2013 and September 2014, samples were collected and analyzed in the Moravian regions of the Czech Republic to isolate the bacterium Escherichia coli. As a result, 212 E. coli isolates were obtained comprising 126 environmental isolates from poultry houses and 86 isolates from cloacal swabs from market-weight turkeys. Subsequently, the E. coli isolates were tested for susceptibility to selected antibiotics. Resistance of the poultry isolates to quinolones ranged from 53% to 73%. Additionally, the presence of plasmid-mediated resistance genes was studied. The genes were confirmed in 58% of the tested strains. The data on resistance of isolates from poultry were compared with results of resistance tests in human isolates obtained in the same regions. The high levels of resistance determined by both phenotyping and genotyping methods and reported in the present study confirm the fact that the use of fluoroquinolones in poultry should be closely monitored.

Keywords: Escherichia coli, antibiotic resistance, poultry, quinolones, plasmids

Received: March 3, 2015; Revised: March 20, 2017; Accepted: March 20, 2017; Published: June 1, 2017  Show citation

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Hricová K, Röderová M, Pudová V, Hanulík V, Halová D, Julínková P, et al.. Quinolone-resistant Escherichia coli in Poultry Farming. Cent Eur J Public Health. 2017;25(2):163-167. PubMed PMID: 28662329.
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    References

    1. Koláčková I, Házová K, Skočková A, Karpíšková R. Occurrence of Shiga toxigenic Escherichia coli strains in pigs and cattle at slaughterhouses in the Czech Republic in 2013. Klin Mikrobiol Infekc Lek. 2014 Jun;20(2):36-9. (In Czech.) Go to PubMed...
    2. Bagheri M, Ghanbarpour R, Alizade H. Shiga toxin and beta-lactamases genes in Escherichia coli phylotypes isolated from carcasses of broiler chickens slaughtered in Iran. Int J Food Microbiol. 2014 May 2;177:1620. Go to original source... Go to PubMed...
    3. Hopkins KL, Davies RH, Threlfall EJ. Mechanisms of quinolone resistance in Escherichia coli and Salmonella: recent developments. Int J Antimicrob Agents. 2005 May;25(5):358-73. Go to original source... Go to PubMed...
    4. Kroemer S, El Garch F, Galland D, Petit JL, Woehrle F, Boulouis HJ. Antibiotic susceptibility of bacteria isolated from infections in cats and dogs throughout Europe (2002-2009). Comp Immunol Microbiol Infect Dis. 2014 Mar;37(2):97-108. Go to original source... Go to PubMed...
    5. Institute for State Control of Veterinary Biologicals and Medicines. Veterinary drugs with restricted indication (2014 update) [Internet]. Prague: Institute for State Control of Veterinary Biologicals and Medicines; 2014 [cited 2017 Mar 20].Available from: http://www.uskvbl.cz/cs/component/search/indika%C4%8Dn%C3%AD%20omezen%C3%AD/?ordering=&searchphrase=all. (In Czech.)
    6. Kolář M, Bardoň J, Sauer P, Kesselová M, Čekanová L, Vágnerová I, et al. Fluoroquinolone-resistant Escherichia coli and Proteus mirabilis in poultry of Middle Moravia, Czech Republic.ActVet Brno. 2005;74(2):249-53. Go to original source...
    7. Martinez M, McDermott P, Walker R. Pharmacology of the fluoroquinolones: a perspective for the use in domestic animals. Vet J. 2006 Jul;172(1):10-28. Go to original source... Go to PubMed...
    8. Robicsek A, Jacoby GA, Hooper DC. The worldwide emergence of plasmid-mediated quinolone resistance. Lancet Infect Dis. 2006 Oct;6(10):629-40. Go to original source... Go to PubMed...
    9. Kučera J. Fluoroquinolones and canine urinary tract infections. Veterinářství. 2006;56(2):71-6. (In Czech.)
    10. Fluit AC, Visser MR, Schmitz FJ. Molecular detection of antimicrobial resistance. Clin Microbiol Rev. 2001 Oct;14(4):836-71. Go to original source... Go to PubMed...
    11. Hooper DC. Emerging mechanisms of fluoroquinolone resistance. Emerg Infect Dis. 2001 Mar-Apr;7(2):337-41. Go to original source...
    12. BrialesA, Rodríguez-Martínez JM, Velasco C, Díaz deAlba P, Domínguez-Herrera J, Pachón J, et al. In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC. Antimicrob Agents Chemother. 2011 Mar;55(3):1266-9. Go to original source...
    13. Cerquetti M, García-Fernández A, Giufrè M, Fortini D, Accogli M, Graziani C, et al. First report of plasmid-mediated quinolone resistance determinant qnrS1 in an Escherichia coli strain of animal origin in Italy. Antimicrob Agents Chemother. 2009 Jul;53(7):3112-4. Go to original source... Go to PubMed...
    14. Ma J, Zeng Z, Chen Z, Xu X, Wang X, Deng Y, et al. High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6)-Ibcr, and qepA among ceftiofur-resistant Enterobacteriaceae isolates from companion and food-producing animals.AntimicrobAgents Chemother. 2009 Feb;53(2):519-24. Go to original source... Go to PubMed...
    15. Skočková A, Koláčková I, Bogdanovičová K, Karpíšková R. Characteristic and antimicrobial resistance in Escherichia coli from retail meats purchased in the Czech Republic. Food Control. 2015 Jan;47:401-6. Go to original source...
    16. European Food Safety Authority; European Centre for Disease Prevention and Control. The European Union Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2012. EFSA J. 2014;12(3):3590. doi:10.2903/j.efsa.2014.3590. Go to original source...
    17. European Centre for Disease Prevention and Control. European Antimicrobial Resistance Surveillance Network (EARS-Net) [Internet]. Stockholm: ECDC [cited 2017 Mar 20]. Available from: http://ecdc.europa.eu/en/healthtopics/antimicrobial-resistance-and-consumption/antimicrobial_resistance/EARS-Net/Pages/EARS-Net.aspx.
    18. Veldman K, Cavaco LM, Mevius D, Battisti A, Franco A, Botteldoorn N, et al. International collaborative study on the occurrence of plasmidmediated quinolone resistance in Salmonella enterica and Escherichia coli isolated from animals, humans, food and the environment in 13 European countries. J Antimicrob Chemother. 2011 Jun;66(6):1278-86. Go to original source... Go to PubMed...
    19. Husičková V, Chromá M, Htoutou Sedláková M, Kolář M. Presence of qnr genes in ESBL-positive isolates of Klebsiella pneumoniae. Klin Mikrobiol Inf Lek. 2012;18(3):60-4. (In Czech.)
    20. Karah N, Poirel L, Bengtsson S, Sundqvist M, Kahlmeter G, Nordmann P, et al.; Norwegian Study Group on PMQR. Plasmid-mediated quinolone resistance determinants qnr and aac(6)-Ib-cr in Escherichia coli and Klebsiella spp. from Norway and Sweden. Diagn Microbiol Infect Dis. 2010 Apr;66(4):425-31. Go to original source... Go to PubMed...
    21. Crémet L, Caroff N, Dauvergne S, Reynaud A, Lepelletier D, Corvec S. Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. Pathol Biol (Paris). 2011 Jun;59(3):151-6. Go to original source... Go to PubMed...
    22. Briales A, Rodríguez-Martínez JM, Velasco C, de Alba PD, RodríguezBaño J, Martínez-Martínez L, et al. Prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6)-Ib-cr in Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain. Int J Antimicrob Agents. 2012 May;39(5):431-4. Go to original source... Go to PubMed...
    23. The European Committee onAntimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 4.0 [Internet]. EUCAST; 2014 [cited 2017 Mar 20]. Available from: https://asmsig.files.wordpress.com/2014/11/breakpoint_table_v_4-01-2014.pdf.
    24. Jacoby GA, Griffin CM, Hooper DC. Citrobacter spp. as a source of qnrB Alleles. Antimicrob Agents Chemother. 2011 Nov;55(11):4979-84. Go to original source... Go to PubMed...
    25. Literak I, Micudova M, Tausova D, Cizek A, Dolejska M, Papousek I, et al. Plasmid-mediated quinolone resistance genes in fecal bacteria from rooks commonly wintering throughout Europe. Microb Drug Resist. 2012 Dec;18(6):567-73. Go to original source... Go to PubMed...
    26. Bardoň J, Kolář M, Karpíšková R, Hricová K. Prevalence of thermotolerant Campylobacter spp. in broilers at retail in the Czech Republic and their antibiotic resistance. Food Control. 2011 Feb 22(2):328-32. Go to original source...
    27. Egervärn M, Börjesson S, Byfors S, Finn M, Kaipe C, Englund S, et al. Escherichia coli with extended-spectrum beta-lactamases or transferable AmpC beta-lactamases and Salmonella on meat imported into Sweden. Int J Food Microbiol. 2014 Feb 3;171:8-14. Go to original source... Go to PubMed...
    28. Leverstein-van Hall MA, Dierikx CM, Cohen Stuart J, Voets GM, van den Munckhof MP, van Essen-Zandbergen A, et al.; National ESBL surveillance group. Dutch patients, retail chicken meat and poultry share the same ESBL genes, plasmids and strains. Clin Microbiol Infect. 2011 Jun;17(6):873-80. Go to original source... Go to PubMed...
    29. Wasyl D, HoszowskiA, Zając M, Szulowski K.Antimicrobial resistance in commensal Escherichia coli isolated from animals at slaughter. Front Microbiol. 2013 Aug 5;4:221. doi: 10.3389/fmicb.2013.00221. Go to original source... Go to PubMed...
    30. Gosling RJ, Clouting CS, Randall LP, Horton RA, Davies RH. Ciprofloxacin resistance in E. coli isolated from turkeys in Great Britain.Avian Pathol. 2012;41(1):83-9. Go to original source... Go to PubMed...
    31. Oosterik LH, Peeters L, Mutuku I, Goddeeris BM, Butaye P. Susceptibility of avian pathogenic Escherichia coli from laying hens in Belgium to antibiotics and disinfectants and integron prevalence. Avian Dis. 2014 Jun;58(2):271-8. Go to original source... Go to PubMed...
    32. van den Bogaard AE, London N, Driessen C, Stobberingh EE.Antibiotic resistance of faecal Escherichia coli in poultry, poultry farmers and poultry slaughterers. J Antimicrob Chemother. 2001 Jun;47(6):763-71. Go to original source... Go to PubMed...
    33. Thorsteinsdottir TR, Haraldsson G, Fridriksdottir V, Kristinsson KG, Gunnarsson E. Broiler chickens as source of human fluoroquinolone-resistant Escherichia coli, Iceland. Emerg Infect Dis. 2010 Jan;16(1):133-5. Go to original source... Go to PubMed...
    34. Thorsteinsdottir TR, Haraldsson G, Fridriksdottir V, Kristinsson KG, Gunnarsson E. Prevalence and genetic relatedness of antimicrobial-resistant Escherichia coli isolated from animals, foods and humans in Iceland. Zoonoses Public Health. 2010 May;57(3):189-96. Go to original source... Go to PubMed...

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