Cent Eur J Public Health 2019, 27(4):340-344 | DOI: 10.21101/cejph.a5000

The presence of multidrug-resistant staphylococcal isolates outside of a major hospital in London, United Kingdom

Adi Idris^1,2, Ron R. Cutler³

¹ PAPRSB Institute of Health Sciences, Brunei Darussalam University, Brunei Darussalam

² Menzies Institute of Health Queensland, School of Medical Science, Griffith University, Southport, Australia

³ School of Biological and Chemical Sciences, Queen Mary University of London, London, United Kingdom

Objective: Drug-resistant staphylococci have been a growing threat to the community and hospitals due to the misuse of antibiotics by humans, industrialisation and lack of novel antimicrobials currently available. Little is known about the prevalence of drug-resistant staphylococci in non-healthcare environments outside hospitals in the London area. Staphylococci can spread via contact with contaminated objects. Traffic light buttons present a fast and easy transmission route for staphylococci.

Methods: Traffic light buttons outside a major hospital in London were swabbed and cultured onto selective media to isolate staphylococci bacteria before performing antimicrobial susceptibility testing on the isolates. The identity of the isolates were determined using MALDI-TOF mass spectrometry (MS). Staphylococci isolates resistant to oxacillin were further tested for minimum inhibitory concentration (MIC). PCR analysis of the mecA gene, a gene that confers resistance to oxacillin, is used to determine the level of resistance to oxacillin.

Results: Eight different staphylococcal species were identified by MALDI-TOF-MS analysis. Out of the 66 staphylococci isolates, 16 were resistant to multiple antibiotics including six isolates which were oxacillin resistant.

Conclusion: This work provides evidence of the presence of multidrug-resistant staphylococci in the vicinity of the hospital environment in London.

Keywords: staphylococci, MALDI, non-hospital, antibiotic resistance, environment

Received: December 7, 2016; Revised: June 30, 2019; Accepted: June 30, 2019; Published: December 31, 2019  Show citation