Cent Eur J Public Health 2023, 31(Suppl 1):S95-S100 | DOI: 10.21101/cejph.a7845

Cytogenetic analysis of coke oven workers in Eastern Slovakia

Vincent Sedlák1, Anton Bujňák2, Andrej Gajdoš2, Dagmar Gajdošová2, Janka Poráčová1, Mária Konečná1, Jozef Fejér3, Daniela Gruľová3, Hedviga Vašková1, Marta Mydlárová Blaščáková1
1 Department of Biology, Faculty of Humanities and Natural Sciences, University of Prešov, Prešov, Slovak Republic
2 Regional Public Health Authority with the Seat in Košice, National Reference Centre for Evaluation of Late Effects of Chemical Substances by Genetic Toxicology Methods, Košice, Slovak Republic
3 Department of Ecology, Faculty of Humanities and Natural Sciences, University of Prešov, Prešov, Slovak Republic

Objective: Our study aimed to evaluate the extent of polycyclic aromatic hydrocarbon (PAH) exposure in coke oven workers from Eastern Slovakia by cytogenetic analysis of human peripheral lymphocytes.

Methods: A total of 81 peripheral blood samples were collected from PAH-exposed workers (mean age 45.84 ± 9.73 years) and 30 samples constituted the control group (41.93 ± 15.39 years). The samples were processed using routine cytological analysis. Conventional cytogenetic analysis of human peripheral lymphocytes has been used to evaluate the effects of PAHs.

Results: Comparison of the aberrant cells in the total exposed with the controls showed a significant difference (p < 0.05). A high level of significance (p < 0.001) was observed when comparing the gaps between the exposed group and the control group. There was a significant difference (p < 0.01) in aberrant cells and chromatid breaks (p < 0.05) in the GR1 working subgroup compared with the control group. The results of the correlation analysis did not show a significant relationship between the length of occupational exposure and the frequency of aberrant cells (r = 0.071, p = 0.529). Similarly, no association was observed between smoking among coke plant workers and the frequency of aberrant cells (r = 0.117, p = 0.538).

Conclusion: Cytogenetic analysis showed an increased frequency of chromosomal aberrations in coke oven workers in Eastern Slovakia.

Keywords: cytogenetic, lymphocytes, coke oven workers, cancer, aromatic hydrocarbons

Received: April 15, 2023; Revised: December 1, 2023; Accepted: December 1, 2023; Published: December 31, 2023  Show citation

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Sedlák V, Bujňák A, Gajdoš A, Gajdošová D, Poráčová J, Konečná M, et al.. Cytogenetic analysis of coke oven workers in Eastern Slovakia. Cent Eur J Public Health. 2023;31(Suppl 1):S95-100. doi: 10.21101/cejph.a7845. PubMed PMID: 38272485.
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