Cent Eur J Public Health 2011, 19(3):131-133 | DOI: 10.21101/cejph.a3635

Serologic Survey of Humans for Flavivirus West Nile in Southern Moravia (Czech Republic)

Zdeněk Hubálek1, Bohumír Kříž2, Jiří Halouzka1
1 Institute of Vertebrate Biology, Academy of Sciences, Brno, Czech Republic
2 National Institute of Public Health, Prague, Czech Republic

A serosurvey for West Nile virus (WNV) was carried out in 525 persons, using a plaque-reduction neutralization microtest (PRNµT) with Vero cells and Egyptian topotype Eg-101 strain as test virus. The blood sera were collected in four South-Moravian districts (Hodonín 44 persons, Břeclav 102 persons, Znojmo 170 persons, Jihlava 209 persons) of the Czech Republic in the years 1988 and 1989, and stored at -20°C. Antibodies to WNV were detected in only three humans (0.6% seropositivity): one person each in the districts of Hodonín (2.3% persons positive), Břeclav (1.0% positive) and Jihlava (0.5% positive), with the titres of 1:64, 1:32, and 1:32, respectively. All the three sera were negative for antibody to tick-borne encephalitis virus. The results indicate that activity of WNV in southern Moravia was very low before 1990.

Keywords: West Nile virus, Flavivirus, Moravia, Czech Republic, serosurvey

Received: August 10, 2010; Revised: June 2, 2011; Accepted: June 2, 2011; Published: September 1, 2011  Show citation

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Hubálek Z, Kříž B, Halouzka J. Serologic Survey of Humans for Flavivirus West Nile in Southern Moravia (Czech Republic). Cent Eur J Public Health. 2011;19(3):131-133. doi: 10.21101/cejph.a3635. PubMed PMID: 22026285.
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