Cent Eur J Public Health 2019, 27(4):312-319 | DOI: 10.21101/cejph.a5216

In vivo assessment of genotoxicity in buccal cells of children undergoing tooth restoration

Lidia Gavić1, Kristina Goršeta2, Domagoj Glavina2, Davor Željezić3, Nada Galić4, Antonija Tadin1
1 Study of Dental Medicine, School of Medicine, University of Split, Split, Croatia
2 Department of Paediatric and Preventive Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
3 Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
4 Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia

Objectives: Whereas dental materials came in direct or close contact with oral tissue, it is a great concern about the biocompatibility of those materials. This study was performed to evaluate possible DNA damage to buccal cells exposed to dental materials.

Methods: This prospective, longitudinal clinical study was conducted over a three months period. Class II restorations were placed in 60 young patients with no previous filling using one of three tested dental materials (two glass ionomers; Ketac Molar and Ionofil Molar and one compomer material; Twinky Star). DNA damage was analysed by micronucleus assays, in buccal exfoliated epithelial cells.

Results: In patients treated with Ketac Molar, a significant frequency of micronuclei (p = 0.027) and binucleated cells in samples taken 30 days following restoration (p = 0.029) was confirmed. In patients treated with Twinky Star, a statistically significant increase in the number of binucleated cells in samples taken after 7 and 30 days following restoration (p = 0.001 and p < 0.001, respectively) was observed. In all samples collected 90 days after treatment, there was no statistical difference in the number of any cell changes.

Conclusion: In this study long-term biocompatibility of tested materials was confirmed. Glass ionomers and compomers are widely used materials in paediatric dentistry, and this study has proved their safety for usage in children.

Keywords: restorative dentistry, dental materials, genotoxicity test, DNA damage, glass ionomer cements, compomers

Received: September 14, 2017; Revised: September 19, 2019; Accepted: September 19, 2019; Published: December 31, 2019  Show citation

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Gavić L, Goršeta K, Glavina D, Željezić D, Galić N, Tadin A. In vivo assessment of genotoxicity in buccal cells of children undergoing tooth restoration. Cent Eur J Public Health. 2019;27(4):312-319. doi: 10.21101/cejph.a5216. PubMed PMID: 31951691.
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